DEPARTMENT OF MOLECULAR BIOTECHNOLOGY AND GENETICS

Head: Renata Kontek, Assoc. Professor

Address:  ul. Banacha 12/13, 90-237, Lodz, building A

 Phone: +48 42 635 44 24

e-mail:

 

Staff:

Name

Phone number

e-mail

building/room

Office hours

Tomasz Sakowicz, Assoc. Professor

+48 42 635 44 28

A/332

Mon
10:00-11:00

Aneta Gerszberg, PhD

+48 42 635 42 19

A/335

Thu
9:30-10:30

Katarzyna Hnatuszko-Konka, PhD

+48 42 635 42 19

A/335

Wed
10:30-11:30

Tomasz Kowalczyk, PhD

+48 42 635 44 27

A/317

Mon
11:30-12:30

Jolanta Krysiak, MSc

+48 42 635 44 23

A/319

-

Tomasz Wiśniak, MSc

+48 42 635 44 23

A/303

-

Danuta Paszkiewicz

+48 42 635 44 23

A/324

-

 

Post-graduate students:

 Name

    Phone number

e-mail

building/room

Office hours

Ewelina Łojewska, MSc

+48 42 635 44 23

A/318

Fri
3:00-4:00
p.m.

 

Research areas

 

Scientific projects / Grants

 

Cooperation

 

Selected publications

  • Gerszberg A., Hnatuszko-Konka K., Kowalczyk T., 2015. In vitro regeneration of eight cultivars of Brassica oleracea var. capitata. In Vitro Cellular & Developmental Biology – Plant 51(1):80-87.
  • Hnatuszko-Konka K., Kowalczyk T., Gerszberg A., Kononowicz A.K., 2014.Phaseolus vulgaris – recalcitrant potential.Biotechnology Advances 32 (7): 1205-15.
  • Kowalczyk T., Gerszberg A., Durańska P., Biłas R., Hnatuszko-Konka K., 2018. High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes. AMB Express 8:125.
  • Sitarek P., Kowalczyk T., Picot L., Michalska-Hejduk D, Bijak M., Białas A.J., Wielanek M., Śliwiński T., Skała E., 2018. Growth of Leonurus sibiricus L. roots with over-expression of AtPAP1 transcriptional factor in closed bioreactor, production of bioactive phenolic compounds and evaluation of their biological activity. Ind. Crops Prod.  122, 732–739
  • Gerszberg A., Hnatuszko-Konka K., Kowalczyk T., Kononowicz AK., 2016. Efficient in vitro callus induction and plant regeneration protocol for different polish tomato cultivars. Not Bot Horti Agrobo 44(2):452-458.
  • Ranoszek-Soliwoda K., Czechowska E., Tomaszewska E., Celichowski G., Kowalczyk T., Sakowicz T., Szemraj J, Grobelny J. Catalase-modified gold nanoparticles: determination of the degree ofprotein adsorption by gel electrophoresis Colloids Surf. B Biointerfaces, 1 (2017), pp. 533-539
  • Olejniczak SA., Łojewska E., Kowalczyk T., Sakowicz T., 2016 Chloroplasts: state of research and practical applications of plastome sequencing. Planta 244:517–527
  • Gerszberg A., Hnatuszko-Konka K., 2017.Tomato tolerance to abiotic stress - a review of most often engineered target sequences. Plant Growth Regul 83:175-198
  • Gerszberg A., 2018. Tissue culture and genetic transformation of cabbage (Brassica oleracea var. capitata): an overview. Planta, 248: 1037-1048,
  • Hnatuszko-Konka K., Łuchniak P., Wiktorek-Smagur A., Gerszberg A., Kowalczyk T.,Gatkowska J., Andrzej K Kononowicz., 2016. The pharmaceutics from the foreign empire: the molecular pharming of the prokaryotic staphylokinase in Arabidopsis thaliana plants
    World Journal of Microbiology and Biotechnology 2016; Vol 32, 7  
  • Grzegorczyk-Karolak I., Wiktorek-Smagur A., Hnatuszko-Konka K., 2018. An untapped resource in the spotlight of medicinal biotechnology: The genus Scutellaria. Biotechnology 19(5):358-371. doi: 10.2174/1389201019666180626105402.
  •  Lojewska E., Kowalczyk T.,  Olejniczak S.,  Sakowicz T., 2016 Extraction and purification methods in downstream processing of plant-based recombinant proteins Protein Expr. Purif., 120  pp. 110-117
  • Weremczuk–Jeżyna I., Grzegorczyk-Karolak I., Frydrych B., Hnatuszko-Konka K., Gerszberg A., Wysokińska H., 2017, Rosmaric acid accumulation and antioxidant potential od Dracocephalum moldavica L. cel suspension culture. Not Bot Horti Agrobo 45(1): 215-219.

 

Selected monographs

·        Wiktorek-Smagur A, Hnatuszko-Konka K, Gerszberg A, Kowalczyk T., 2015. Arabidopsis thaliana transformation - a Little weed with thousand faces. In Feagaiga C. (ed) Arabidopsis thaliana: cultivation, life cycle and functional genomics. NOVA Science Publishers, Inc. (NOVA).

·        Wiktorek-Smagur A., Hnatuszko-Konka K., Gerszberg A., Kowalczyk T., Łuchniak P., Kononowicz A.K. 2012. Green way of biomedicine – How to force plants to produce new important proteins. Transgenic Plants – Advances and Limitations, Yelda Ozden (Ed.), ISBN 978-953-307-953-0, InTech

 

 

Theses topics

Master’s theses

 

Bachelor’s theses

 

LABORATORY OF CYTOGENETICS  (in DEPARTMENT OF  GENERAL GENETICS, PLANT MOLECULAR BIOLOGY AND BIOTECHNOLOGY)

Head: Renata Kontek, Assoc. Professor 

Address:  ul. Banacha 12/13, 90-237, Lodz, building A

Phone: +48 42 635 44 24

Fax: +48 42 635 44 23

e-mail:

 

Staff:

Name

Phone number

e-mail

building/room

Office hours

Renata Kontek, Assoc. Professor

+48 42 635 44 24

A/314

Mon
2:00-3:00 p.m.

Regina Osiecka, retired Professor 

-

-

-

Alina Błaszczyk, Assoc. Professor

+48 42 635 44 26

A/308

Thu
1:00-2:00 p.m.

Beata Marciniak,
PhD

+48 42 635 44 26

A/ 334

Tue
11:00-12:00

 

Post-graduate students:

 Name

Phone number

e-mail

building/room

Office hours

Gabriela Gajek, MSc

+48 42 635 44 26

A/334

Thu
1:30-2:30 p.m.

Karol Bukowski, MSc

+48 42 635 44 26

A/318

-

 

Research areas

    • research on biological activity  of compound with potential anticancer activity  with the use of cancer cell lines and human lymphocytes
    • research on biological activity of   antioxidative compounds
    • research on genotoxicity and cytotoxicity of pesticides using plant material

 

Cooperation

    • Laboratory of Endoscopy of Gastrointestinal Tract,  Władysław Biegański Lodz Specialistic Hospital,   
    • Laboratory of Molecular Bases of Medicine, Medical University
    • Department of Organic Chemistry, Universityof Lodz
    • Department of General Biochemistry, University of Lodz
    • Laboratory of Bioinorganic Chemistry Medical Universiry of Lodz

 

Selected publications

 

  • Kontek R., Nowicka H., 2013, The modulatory effect of melatonin on genotoxicity of iriontecan in normal lymphocytes and cancer cells, Drug and Chemical Toxicology 36(3): 335 – 342.
  • Kontek R., Kontek B. and Grzegorczyk K., 2013, Vitamin C modulates DNA-damage induced by hydrogen peroxide in the human colorectal adenocarcinoma cell lines (HT29) estimated by comet assay in vitro, Archives of Medical Science 6: 1006-1012.
  • Błaszczyk A., Augustyniak A., Skolimowski J., 2013, Ethoxyquin: An Antioxidant Used in Animal Feed, International Journal of Food Science, article ID 585931, 12 pages; DOI: 10.1155/2013/585931
  • Marciniak B., Łopaczyńska D., Kowalczyk E., Skośkiewicz J., Witczak M., Majczyk M., Grabowicz W., Ferenc T., 2013, Evaluation of micronuclei in mice bone marrow and antioxidant systems in erythrocytes exposed to α-amanitin.; Toxicon 63: 147-153
  • Kontek R., Jakubczak M., Matławska-Wasowska K., 2014, The antioxidants, vitamin A and E but not vitamin C and melatonin enhance the proapoptotic effects of irinotecan in cancer cells in vitro, Toxicology in Vitro 28, 282-291.
  • Nowak M., Malinowski Z., Jóźwiak A., Fornal E., Błaszczyk A., Kontek R., 2014, Substituted benzoquinazolinones. Part 1: Synthesis of 6-aminobenzo[h] quinazolinones via Buchwald-Hartwig amination from 6-bromobenzo[h] quinazolinones, Tetrahedron 70, 5153-5160.
  • A.A. Klimczak, A. Matusiak, J. Lewkowski, J.Bitner, J. Szemraj, R. Kontek, 2015, Dimethyl (2-furyl)-N-(2-methoxyphenyl)aminomethylphosphonate Induces Apoptosis in Esophageal Squamous Cancer Cells. Structure vs. Activity of Its Selected Analogs, Phosphorus, Sulfur, and Silicon and the Related Elements, Phosphorus, Sulfur, and Silicon 190:1088–1099.
  • Z. Malinowski, E. Fornal, M. Nowak, R. Kontek, G. Gajek, B. Borek, 2015, Synthesis and biological evaluation of some amino- and sulfanyl- 3H-quinazolin-4-one derivatives as potential anticancer agents, Monatshefte fur Chemie - Chemical Monthly, 146:1723–1731.
  • Błaszczyk A., Skolimowski J., 2015, Cytotoxicity and genotoxicity of ethoxyquin used as an antioxidant, Food Reviews International, 31: 222-235
  • Plak A., Gajek G., Błaszczyk A. 2015, Aneuploidy of chromosome 21: the most important risk factors, Postępy Biologii Komórki 4
  • B. Marciniak i wsp., 2015, „Zagrożenia zdrowia publicznego. Zdrowie człowieka                         a środowisko. Część 2”. red. Andrzej Denys, wyd. Wolters Kluwer S.A. Warszawa
  • Nowak M., Malinowski Z., Jóźwiak A., Kontek R., 2015, Substituted benzoquinazolinones. Part 2: Synthesis of 6-aminobenzo[h] quinazolinones via Buchwald-Hartwig amination from 6-bromobenzo[h] quinazolinones, Tetrahedron 7: 9463-9473
  • Lewkowski J., Moya M.R., Wrona-Piotrowicz A., Zakrzewski J., Kontek R. and Gajek G., 2016, Synthesis, fluorescence properties and the promising cytotoxicity of pyrene–derived aminophosphonates, Beilstein J. Org. Chem., 12, 1229–1235.
  • K. Adach, M. Fijalkowski, G. Gajek, J. Skolimowski, R. Kontek, A. Blaszczyk, Studies on the cytotoxicity of diamond nanoparticles against human cancer cells and lymphocytes, Chemico-Biological Interactions, Volume 254, 25 July 2016, Pages 156-166

Theses topics

Master’s theses

  • The influence of a compound or of a group of compounds on DNA danae induction in selected cancer cell lines.                                                                  
  • Analysis of the biological properties of New compounds with potential anticancer activity
  • Genotoxicity and cytotoxicity analyses (human material: cancer cell lines, peripherial blond lymphocytes or plant material).  
  • Induction of apoptosis and necrosis in human cancer cells after the treatment with the tested compounds.

Bachelor’s theses

  • Fragile X chromosome
  • Common fragile sites
  • Mucoviscidisis
  • Heritable fragile sites
  • Chromosom territory   
  • Disease associated with premature aging adult – Werner syndrome
  • X-chromosome linked disease: Incontinentia pigmenti  (Bloch-Sulzberger syndrome)
  • The importance of cohesion complex in the formation of trisomy 21
  • Duchenne and Becker muscular dystrophy
  • Genetic determinants in Alzheimer’s disease
  • Prenatal diagnostics of development al and genetic anomalies.
  • Klinefelter syndrome
  • Disease associated with premature aging - progeria
  • Sickle cell anemia
  • Philadelphia chromosome
  • Numerical chromosomal aberrations
  • Human diseases caused by chromosomal  structural abnormalities
  • DiGeorge syndrome
  • Cisplatin in cancer chemotherapy

 

Photo Gallery

 

 

Pictures of lung cancer apoptotic cells stained with enthidinum bromide and acridine orange (A Kluka’s Master’s thesis, 2010).

 

 

 

Cytotoxicity assessment with MTT test (picture of plates, A. Iwaniuk’s  Master’s thesis ,2011)

                 

 

 

Pictures of comets after treatment of colon cancer cells with SN-38 (H.Machałowska’s Master’s thesis, 2011)

 

 

·        Gerszberg A., Hnatuszko-Konka K., Kowalczyk T., 2015. In vitro regeneration of eight cultivars of Brassica oleracea var. capitata. In Vitro Cellular & Developmental Biology – Plant 51(1):80-87.

·        Hnatuszko-Konka K., Kowalczyk T., Gerszberg A., Kononowicz A.K., 2014.Phaseolus vulgaris – recalcitrant potential.Biotechnology Advances 32 (7): 1205-15.

·        Kowalczyk T., Gerszberg A., Durańska P., Biłas R., Hnatuszko-Konka K., 2018. High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes. AMB Express 8:125.

·        Sitarek P., Kowalczyk T., Picot L., Michalska-Hejduk D, Bijak M., Białas A.J., Wielanek M., Śliwiński T., Skała E., 2018. Growth of Leonurus sibiricus L. roots with over-expression of AtPAP1 transcriptional factor in closed bioreactor, production of bioactive phenolic compounds and evaluation of their biological activity. Ind. Crops Prod.  122, 732–739

·        Gerszberg A., Hnatuszko-Konka K., Kowalczyk T., Kononowicz AK., 2016. Efficient in vitro callus induction and plant regeneration protocol for different polish tomato cultivars. Not Bot Horti Agrobo 44(2):452-458.

·        Ranoszek-Soliwoda K., Czechowska E., Tomaszewska E., Celichowski G., Kowalczyk T., Sakowicz T., Szemraj J, Grobelny J. Catalase-modified gold nanoparticles: determination of the degree ofprotein adsorption by gel electrophoresis Colloids Surf. B Biointerfaces, 1 (2017), pp. 533-539

·        Olejniczak SA., Łojewska E., Kowalczyk T., Sakowicz T., 2016 Chloroplasts: state of research and practical applications of plastome sequencing. Planta 244:517–527

·        Gerszberg A., Hnatuszko-Konka K., 2017.Tomato tolerance to abiotic stress - a review of most often engineered target sequences. Plant Growth Regul 83:175-198

·        Gerszberg A., 2018. Tissue culture and genetic transformation of cabbage (Brassica oleracea var. capitata): an overview. Planta, 248: 1037-1048,

·        Hnatuszko-Konka K., Łuchniak P., Wiktorek-Smagur A., Gerszberg A., Kowalczyk T.,Gatkowska J., Andrzej K Kononowicz., 2016. The pharmaceutics from the foreign empire: the molecular pharming of the prokaryotic staphylokinase in Arabidopsis thaliana plants
World Journal of Microbiology and Biotechnology 2016; Vol 32, 7  

·        Grzegorczyk-Karolak I., Wiktorek-Smagur A., Hnatuszko-Konka K., 2018. An untapped resource in the spotlight of medicinal biotechnology: The genus Scutellaria. Biotechnology 19(5):358-371. doi: 10.2174/1389201019666180626105402.

·         Lojewska E., Kowalczyk T.,  Olejniczak S.,  Sakowicz T., 2016 Extraction and purification methods in downstream processing of plant-based recombinant proteins Protein Expr. Purif., 120  pp. 110-117

·        Weremczuk–Jeżyna I., Grzegorczyk-Karolak I., Frydrych B., Hnatuszko-Konka K., Gerszberg A., Wysokińska H., 2017, Rosmaric acid accumulation and antioxidant potential od Dracocephalum moldavica L. cel suspension culture. Not Bot Horti Agrobo 45(1): 215-219.