DEPARTMENT OF IMMUNOLOGY AND INFECTIOUS BIOLOGY

Head of the Department : prof. dr hab. Magdalena Mikołajczyk-Chmiela

Address: 90-237 Łódź, ul. Banacha 12/16, building A

Phone: +48 42 635 41 86

Fax: +48 42 665 58 18

e-mail:

Adress/link 

Department of Imunoparasitology

Staff:

Name and surname

Phone

e-mail

Building/room

Consultation hours

dr hab. Bożena Dziadek (Univ. Prof.) – Head of the Department

+48 42 635 46 66

A/279

Fr. 12:00-14:00

prof. dr hab. Henryka Długońska

+48 42 635 45 26

A/271

Wed. 12:00-14:00

dr Katarzyna Dzitko (Univ. Prof.)

+48 42 635 46 66

A/279

Fr. 12:00-14:00

dr Justyna Gatkowska (adjunct)

+48 42 635 46 66

A/276

Fr. 12:00-14:00

 

PhD students:

Name and surname

Phone

e-mail

Building/room

 Consultation hours

mgr Malwina Kawka

+48 42 635 46 66

A/276  Fr. 12:00-14:00

mgr Adrian Bekier

+48 42 635 46 66

A/276 Fr. 12:00-14:00

 
 

 

Department of Cellular Immunology

Staff:

Name and surname

Phone

e-mail

Building/room

Consultation hours

dr Marek Fol (Univ. Prof.) – Head of the Department

+48 42 635 44 72

 

A/392

Mon. 10:00-12:00

prof. dr hab. Wiesława Rudnicka (professor emeritus)

 

A/394

 

dr hab. Magdalena Druszczyńska (adjunct)

+48 42 635 44  70

A/393

Wed. 10:00-12:00

Dr Magdalena Kowalewicz-Kulbat (adjunct)

+48 42 635 44 70

A/393

Wed. 10:00-12:00

Dr Marcin Włodarczyk (adjunct)

+48 42 635 44 71

A/392

Wed. 10:00-12:00

 

PhD students:

Name and surname

Phone

e-mail

Building/room

 Consultation hours

mgr Sebastian Wawrocki

+48 42 635 44 71

 A/353  Mon. 9:00-11:00

mgr Krzysztof Krawczyk

+48 42 635 44 71

 A/353  

 

Laboratory of Infectious Biology

Staff:

Name and surname

Phone

e-mail

Building/room

Consultation hours

dr hab. Beata Sadowska (univ. Prof.) – Head of the Laboratory

+48 42 635 45 25

A/274

Wed. 11:00-13:00

prof. dr hab. Barbara Różalska

+48 42 635 41 85

A/273

Thu. 11:30-13:30

dr M. Więckowska-Szakiel (research techn.)

+48 42 635 45 25

A/274

 

 

PhD students:

Name and surname

Phone

e-mail

Building/room

 

Consultation hours
         

 

Laboratory of Gastroimmunology

Staff:

Name and surname

Phone

e-mail

Building/room

Consultation hours

prof. dr hab. Mikołajczyk- Chmiela – Head of the Laboratory

+48 42 635 41 86

A/391

Thu. 12:00-14:00

dr Agnieszka Matusiak (research techn.)

+48 42 635 45 41

A/278

 

dr Maria Walencka (lecturer)

+48 42 635 45 41

A/278

Mon. 10:15-12:15

dr Karolina Rudnicka (adjunct)

+48 42 635 45 41

A/278

Mon. 10:15-12:15

PhD students:

Name and surname

Phone

e-mail

Building/room

 

Consultation hours

mgr Adrian Gajewski +48 42 639 51 60

 

A/264 Fr. 10:00-12:00
mgr Weronika Gonciarz +48 42 639 51 60 A/264 Mon. 12:00-14:00

 

 

Research topics

 

Department of Cellular immunology:

Genetic and immunological reasons of resistance and susceptibility of people to tuberculosis, diagnostic markers of tuberculosis; effectiveness of BCG vaccination in the development of late hypersensitivity to tuberculin; importance gene polymorphisms for defense functions of macrophages, the expression of receptors that recognize molecular patterns of pathogens, production of cytokines, the use of recombinant strains of atenuated Mycobacterium tuberculosis in understanding the molecular basis of intracellular persistence of mycobacteria within macrophages, selecting of targets for antituberculous drugs; regulatory role of dendritic cells in allergic diseases, the attempt to use the BCG vaccine to achieve the Th2/Th1 cellular balance in the patients with asthma and rhinitis.

 

Department of Immunoparasitology

Interactions of parasitic protozoan Toxoplasma gondii with host proteins; effect of prolactin and other hormones  (e.g. ecdyson) on the development of Toxoplasma infection; generation of  recombinant T. gondii antigens and assessment of their diagnostic utility; construction and evaluation of immunogenic and protective activity of novel DNA- or recombinant protein-based vaccines against T. gondii on a mouse experimental model; in vitro testing of newly synthesized compounds (thiadiazoles, thiosemicarbazides etc.) for their anti-toxoplasmic activity.

Laboratory of Infectious Biology

Conducted research aimed to complement and deepen the knowledge of bacterial and fungal biofilms and the search for new therapeutic strategies that could support modern chemotherapy to combat these complex structures of microorganisms. We seek to answer the following questions: What mechanisms determine the high resistance of biofilms of S. aureus and C. albicans to antibiotics / antimycotics and mechanisms of humoral immunity / host cell ? Whether and to what extent natural factors produced by prokaryotic cells (bacteria) or eukaryotic (plant, animal) may be active against biofilms and what is their mechanism of action ? Whether is possible to achieve their synergistic anti-biofilm effect? Do such natural products can operate supportively on host defenses against infections with a biofilm nature? Whether and what innovative drug delivery strategies will be effective in the fight against biofilms which are still serious medical problems ?

Laboratory of Gastroimmunology:

The main topics: Gastritis and peptic ulcer of the stomach and duodenum as a consequence of Helicobacter pylori infection . The role of H. pylori in the development of coronary heart disease. Genetic and immunological reasons of resistance and susceptibility of humans to infection with H. pylori,  variable course of such infections, the role of H. pylori antigens in the initiation and modulation of immune processes - natural innate and acquired specific: phagocytosis, NK cells, T lymphocytes, cellular cytotoxic activity, proliferation and secretory responses, phenotyping of the immune cells, assessment of the effects of antigenic mimicry resulting from the similarity of H. pylori components and the host cells. Cell signaling via receptors for pathogen molecular patterns and cytokines; polymorphisms of the genes encoding such receptors. Humoral immune response. Diagnostic determinants of H. pylori infections; effectiveness of non-invasive tests. Searching for new therapies, alternative to antibiotics. Development of animal models of H. pylori infections to explore natural history and the mechanisms of H. pylori infections.

 

Research projects
Infective endocarditis – the in vitro biomedical analysis of the potential therapeutic properties of selected phytochemicals (National Center of Science, 2014-2016)

Determinants of inflammatory and immune responses in guinea pigs infected with Helicobacter pylori (National  Center of Science, Poland, 2014-2016)

The immunogenic and protective activity of recombinant Toxoplasma gondii ROP5 and ROP18 proteins (National Center of Science, Poland, 2012-2015)

Biomedical analysis of alternative therapy of chronic wound infections. In vitro research with plant polyphenols (National Center of Science, 2013-2014)

Immunogenicity and neuroprotectivity evaluation of recombinant vaccines against toxoplasmosis in an experimental murine model (National Center of Science, Poland, 2011-2014)

Phytoecdysteroids as potential medicines inhibiting the development of Toxoplasma gondii infection (National Center of Science, Poland, 2011-2014)

Assessment of virulence, biofilm formation and drug-susceptibility of Candida albicans treated with plant essential oils. Potential use of oils in combination with medicines in therapy of chronic wound infections with fungal-bacterial etiology (National  Center of Science, Poland, 2011-2013)

Comprehensive research of interaction of selected acute phase proteins with the cells and antigens of Mycobacterium (2011-2012), the European Regional Development Fund

Selective suppression of alergen-specific Th2 response by vaccine M. bovis BCG bacilli (Polish Ministry of Science and Higher Education,  2009- 2012)

Active vinylphosphonates – a new application in the synthesis of biologically important heterocyclic forms and alpha-aminophosphonian acids (Polish Ministry of Science and Higher Education, 2009-2012, collaboration with the leading center: Faculty of Chemistry, Technical University in Łódź, Poland)

Factors affecting the survival of M. tuberculosis in the mononuclear phagocytic cells of the host (Polish Ministry of Science and Higher Educationn, 2008-2011)

Impact of gastric pathogens on the secretion of enterohormones (grelin, insulin, leptin, neuropeptide Y, peptide YY, orexin and alpha MSH) in children with growth deficiencies: the phenomenon of molecular mimicry (National Center of Science, Poland, 2010-2013, collaboration with the leading center: Institute Polish Mother Health Center, Łódź, Poland)

The development of the guinea pig model to evaluate the properties of biological compounds enhancing of resistance to infectious diseases in humans and animals (National Center of Science, Poland, 2010-2013)

Immunological reactions to virulent and attenuated mycobacteria as the base of the optimization of diagnosing and prevention of this disease(National Center of Science, Poland, 2010-2013)

Cellular and humoral cytotoxic reactions in H. pylori infections (Polish Ministry of Science and Higher Education, 2009-2012)

The role of prolactin (PRL) in human immunity against Toxoplasma gondii (Polish Ministry of Science and Higher Education, Poland, 2008-2010)

Staphylococcal biofilms in confrontation with host immune cells and antibiotics. Why do we lose? (Polish Ministry of Science and Higher Education , 2008-2010)

The role of H. pylori LPS and the host receptors: CD14 and TLR4 in the course of H. pylori related diseases – gastric ulcer disease and coronary heart disease(Polish Ministry of Science and Higher Education, 2006-2008)

Polymorphism of genes regulating anti-mycobacterial properties of macrophages in health individuals and patients with tuberculosis (Polish Ministry of Science and Informatics, 2006-2007)

Assessment of biological activity of natural antibacterial compounds against staphylococcal biofilm. Own methodology (Polish Ministry of Science and Informatics, 2005-2007)

Interactions of H. pylori ligands with soluble host receptors in children and adults with chronic dyspepsia (The Committee of Scientific Research, Poland 2003-2005)

Noninvasive diagnostics tests for detection of H. pylori infections – development of own methodological solution and evaluation of selected commercial assays (The Committee of Scientific Research, Poland, 2000-2001)

Specific cellular immune reactions as markers enabling prediction of susceptibility or resistance to H. pylori infections (The Committee of Scientific Research, Poland, 1998-1999)  

Phenotypic variation of H. pylori strains and clinical course of peptic ulcer disease, nonulcer dyspepsia (NUD) or asymptomatic infection in children and adolescents (The Committee of Scientific Research, Poland, 1995-1997)  

 

Cooperation

- Institute Center of Polish Mother Health in Łódź, Ist Clinic of Pediatry and Gastroenterology, Poland

- Institute Center of Polish Mother Health in  Łódź,  Clinic of Pediatry and Immunology  with Nephrology Unit, Poland

- Medical University in Łódź, Poland, II Cardiology Clinic, Hospital named of Wł. Biegański Medical University in Łódź, Poland,

- Department of Internal Medicine and Clinical Pharmacology, Hospital  named of Wł. Biegański

- Medical University in Łódź, Poland, Department of Pharmaceutical Biochemistry

- Medical University in Łódź, Poland, Department of Coagulopathy

- Laboratory of Mycobacterium Biology, Łódź, Poland

- Technical University of Łódź, Poland, Faculty of Biotechnology and Food Sciences, Institute of Technical Biochemistry

- Centre of Medical Biology, Polish Academy of Sciences, Łódź, Poland

- University Clinical Hospital. Military Medical Academy, Medical University Central Hospital of Veterans, Cardiology and Endocrinology Units, Łódź, Poland

- Medical University of Łódź, Poland, Department of Immunology, Rheumatology and Allergy

- Medical University of Łódź, Poland, Department of Immunoendocrinology

- Public Unit of Health Care, Center for the Treatment of Lung Diseases and Rehabilitation, Division of Tuberculosis Treatment, Tuszyn, Poland

- UMR 8576 Université des Sciences et Technique de Lille, Lille, France

- UR918, INRA  Centre de Tours, Nouzilly, France

 

Exchange of students:

Travels abroad:
UREKA – Ireland (1 person)
IAESTE – Serbia (2 persons); Germany (1 person); Colombia (2 persons); Switzerland (1 person)

Visits of foreign students : IAESTE – Croatia (1 person); Great Britain (2 persons); Spain (1 person); Bosnia  and Herzegovina (1 person), Germany (1 person), Switzerland (1 person)

 

Services/Expertise

Cooperation with industry:

- microbiological purity of cosmetics

- testing of antimicrobial properties of cosmetic products and their ingredients

- evaluation of antimicrobial properties of functional textiles and textile products

- testing and evaluation of the effectiveness of preservation of cosmetic products by various methods (PN-EN ISO 11930, KOKO Test Polish Pharmacopoeia Ed. IX)

 

Selected publications

1. Dzitko K., Lawnicka H., Gatkowska J., Dziadek B., Komorowski J., Długońska H. 2012. Inhibitory effect of prolactin on Toxoplasma proliferation in peripheral blood mononuclear cells from patients with hyperprolactinemia. Parasite Immunology34: 302-311.

2. Dziadek B., Gatkowska J., Grzybowski M., Dziadek J., Dzitko K., Dlugonska H. 2012. Toxoplasma gondii: The vaccine potential of three trivalent antigen-cocktails composed of recombinant ROP2, ROP4, GRA4 and SAG1 proteins against chronic toxoplasmosis in BALB/c mice. Experimental Parasitology 131, 133-138.

3. Gatkowska J., Wieczorek M., Dziadek B., Dzitko K., Dlugonska H. 2012. Behavioral changes in mice caused by Toxoplasma gondii invasion of brain. Parasitology Research111, 53-58.

4. Kuźma Ł., Wysokińska H., Różalski M., Budzyńska A., Więckowska-Szakiel M., Sadowska B., Paszkiewicz M., Kisiel W., Różalska B. 2012. Antimicrobial and anti-biofilm properties of new taxidione derivative from hairy roots of Salvia austiaca. Phytomed. http://dx.doi.org/10.1016/j.phymed.2012.07.016.

5. Budzisz E., Bobka R., Hauss A., Roedel J.N., Wirth S., Lorenz I.P., Różalska B., Więckowska-Szakiel M., Krajewska U., Różalski M. 2012. Synthesis, structural cvharacterization, antimicrobial and cytotoxic effects of aziridine, 2-aminoethylaziridine and azirine complexes of copper (II) and palladium (II). Dalton Transactions, doi: 10.1039/c2dt12107g.

6. Rudnicka K., Włodarczyk M., Moran A. P., Rechciński T., Miszczyk E., Matusiak A., Szczęsna E., Walencka M., Rudnicka W., Chmiela M. 2012. Helicobacter pylori antigens as potential modulators of lymphocyte’ cytotoxic activity. Microbiol. Immunol. 56: 62-75.

7. Miszczyk E., Rudnicka K., Moran A.P., Fol M., Kowalewicz-Kulbat M., Druszczyńska M., Matusiak A., Walencka M., Rudnicka W., Chmiela M. 2012. Interaction of helicobacter pylori with C-type lectin dendritic cell – specific ICAM grabbing nonintegrin. J. Biomed. Biotechnol. ID 206463, doi:10.1155/2012/206463.

8. Dziadek B., Gatkowska J., Brzostek A., Dziadek J., Dzitko K., Grzybowski J., Długońska H. 2011. Evaluation of three recombinant multi-antigenic vaccines composed of surface and secretory antigens of Toxoplasma gondii in murine models of experimental toxoplasmosis. Vaccine 29, 821-830.

9. Fol M., Zawadzka K., Druszczyńska M., Kowalewicz-Kulbat M., Rudnicka W. 2011. Vaccination against M. tuberculosis – what next after BCG? Post. Hig. Med. Dośw. 65: 93-103.

10. Fol M., Olek J., Kowalewicz-Kulbat M., Druszczyńska M., Rudnicka W. 2011. Prątki niegruźlicze: M. marinum, M. ulcerans, M. xenopi- krótka charakterystyka drobnoustrojów
i zmian klinicznych przez nie wywołanych (Non-tuberculous mycobacteria: M. marinum, M. ulcerans, M. xenopi – short characteristic of microbes and clinical disorders) Post. Hig. Med. Dośw. 65: 574-583.

11. Druszczyńska M., Włodarczyk M., Fol. M., Rudnicka W. 2011. Rozpoznawanie antygenów prątków przez fagocyty Recognition of mycobacterial antigens by phagocytes). Post. Hig. Med. Dośw. 65: 28-39.

12. Budzyńska A., Różalski M., Karolczak W., Więckowska-Szakiel M., Sadowska B., Różalska B. 2011. Synthetic 3-arylideneflavanones as inhibitors of the initial stages of biofilm formation by Staphylococcus aureus and Enterococcus faecalis. Z. Naturforsch. 66c, 104-114.

13. Budzyńska A., Więckowska-Szakiel M., Sadowska B., Kalemba D., Różalska B. 2011. Antibiofilm activity of selected plant essential oils and their major components. Pol. J. Microbiol. 60, 35-41.

14. Dzitko K., Gatkowska J., Płociński P., Dziadek B., Długońska H. 2010. The effect of prolactin (PRL) on the growth of Toxoplasma gondii tachyzoites in vitro. Parasitol. Res. 107, 199-204.

15. Szczepańska M., Włodarczyk M., Janiszewska Drobińska B., Kielnierowski G., Zawadzka J., Fol M., Rudnicka W., Druszczyńska M. 2010. Makrofagowe receptory w gruźlicy (The macrophage receptors in tuberculosis). SEPSIS 4 (3): 209-213.

16. Sadowska B., Walencka E., Więckowska-Szakiel M., Różalska B. 2010. Bacteria competing with the adhesion and biofilm formation by Staphylococcus aureus. Folia Microbiol. 52.

17. Grebowska A., Moran A. P., Bielański W., Matusiak A., Rechciński T., Rudnicka W., Baranowska A., Rudnicka W., Chmiela M. 2010. Helicobacter pylori lipopolysaccharide activity in human peripheral blood mononuclear leukocyte cultures. J Physiol. Pharmacol. 61: 437-442.

18. Kowalewicz-Kulbat M., Kaźmierczak D., Donevski S., Biet F., Pestel J., Rudnicka W. 2008. Naive helper T cells from BCG-vaccineted volunteers produce IFN-g and IL-5 to mycobacterial antigen-pulsed dendritic cells. Folia Histochemica et Cytobiologica, vol. 46, No 2.

19. Chmiela M., Michetti P. 2006. Inflammation, immunity, vaccines for Helicobacter pylori infection. Helicobacter 11 (suppl. 1): 21-26.

20. Basińska B., Wiśniewska M., Chmiela M. 2004. Principle of a new immunoassay based on electrophoretic mobility of poly(styrene/a-tert-butoxy-w-vinylbenzyl-polyglycidol) microspheres: application for the determination of Helicobacter pylori IgG in blood serum. Macromol. Biosc. 5: 70-77.

21. Paziak-Domańska B., Chmiela M., Jarosińska A., Rudnicka W. 2000. Potential role of CagA in the inhibition of T cell reactivity in Helicobacter pylori infections. Cell. Immunol. 202: 136-139.

 

Dissertation topics

Bachelor’s degree  dissertations:

Students prepare theoretical theses on immunology, immunology of infection, clinical microbiology, microbiology, sanitary microbiology, microbiological diagnostics, biotechnology, cosmetics, medical biotechnology, using the scientific literature in Polish and English. They develop the topic chosen by themselves, or suggested by a tutor, collect literature in the field, analyze research problems and formulate conclusions. They write the thesis according to established guidelines and supply an electronic version as well. They present their thesis during seminar, in conjunction with a multimedia presentation.

Examples of dissertations for bachelor degree

  1. Morphological forms of Helicobacter pylori  - the potential role in infection transmission.
  2. Biostimulators of the immune response of the host.
  3. Antibacterial  cationic proteins of the host: catepsins and catelucidins – structure and functions.
  4. Biomarkers of Mycobactrium  tuberculosis infections.
  5. Experimental Trypanosoma cruzi invasion therapies.
  6. Trichomonas - human parasite or commensal.
  7. The role of apoptosis in physiological processes and infections.
  8. Microbial biofilms. Their importance in the environment and the maintenance of human health.

 Master’s degree dissertations:

Master's thesis includes experimental studies in the field of immunology, immunology of infection, clinical microbiology, general microbiology, sanitary microbiology, microbiological diagnostics, biotechnology, cosmetics  medical biotechnology. The works are carried out within the framework of research projects in the unit, under the direct supervision of faculty staff. Students collect scientific literature that will help them to formulate a research hypothesis and carry out  experimental work. They perform experiments, evaluate the results and analyze them in terms of statistics as well as conduct scientific discussion. Then they write thesis on the basis of the guidelines and provide its electronic version. Students present their diploma work during master degree seminar using the presentation in Power Point .

Examples of dissertations for master degree:

  1. Modulation of H. pylori colonization in guinea pigs by M. bovis BCG.
  2. Specificity of interaction of H. pylori LPS DC-SIGN receptor.
  3. The role of IL-8 in the interaction of macrophages with Mycobacterium tuberculosis investigated on the level of phagocytosis and the secretion of cytokines.
  4.  Variants of IL-10 gene and the production of IL-10 in the cultures of the whole blood cells from the patients with active or latent tuberculosis, stimulated with mycobacterial antigens.
  5. Preparation of recombinant Toxoplasma gondii MAG1 antigen using E. coli expression system.
  6. Cytotoxic activity of peripheral blood NK cells towards HeLa target cells in response to   H. pylori LPS.
  7. Secretory activity of mice macrophages stimulated with the culture supernatants of Staphylococcus areus growing in suspension or as biofilm.
  8. Estimation of antibacterial activity of selected vegetable oils and their ingredient against clinical strains of Candida spp.

 

Materials for students

Useful links

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