DEPARTMENT OF CYTOPHYSIOLOGY

Head: Janusz Maszewski, Professor 

Address:  Pomorska 141/143, 90-236 Lodz, building D

Phone: +48 42 635 45 12

Fax: +48 42 635 45 14

e-mail:

 

Staff:

Name

Phone number

e-mail

building/room

Office hours

Janusz Maszewski, Professor 

+48 42 635 45 12

D / cyt-9

Fri
11:00-1:00
p.m.

Maria Kwiatkowska, retired Professor

+48 42 635 47 79

D/cyt-12

-

Andrzej Kaźmierczak, Assoc. Professor

+48 42 635 42 03

D/cyt-5

Wed
10:00-11:30

Justyna Polit,
Assoc. Professor

+48 42 635 47 36

D/cyt-11

Wed
11:00-12:30

Katarzyna Popłońska,
Assoc. Professor

+48 42 635 47 34

D/cyt-6

Thu
12:00-1:30
p.m.

Dariusz Stępiński, Assoc. Professor

+48 42 635 47 34

D/cyt-6

Wed
1:00-2:30
p.m.

Dorota Rybaczek, Assoc. Professor

+48 42 635 47 35

D/cyt-8

Tue
1:00-2:30
p.m.

Agnieszka Wojtczak, PhD

+48 42 635 47 34

D/cyt-6

Mon
1:30-3:00
p.m.

Konrad Winnicki, PhD

+48 42 635 45 14

D/cyt-4

Mon
1:00-2:30 p.m.

Aneta Żabka, PhD

+48 42 635 47 36

D/cyt-11

-

Dorota Kasprzak, MSc

+48 42 635 45 14

D/cyt-7

-

Marta Gabryelczyk, MSc

+48 42 635 45 14

D/cyt-7

-

 

Post-graduate students:

 

Name                      

 Phone number                

e-mail

 building/room        

Office hours

 

       

 

  • Research areas
    • the role of DNA topoisomerase in DNA replication and mitosis in plants
    • the role of late heterochromatin replication in specification of transcriptional activity
    • signal transduction during induction and premature chromosome condensation
    •  ability of PCC chromatin to continue replication in plant and animal cells
    • ultrastructural and immunocytochemical studies of Chara spermiogenesis
    • influence of  topoisomerase II inhibitors  on spermiogenesis
    • the role of ER in Chara spermiogenesis
    •  induction of asynchronous chromatin condensation under replication stress
    •  structure and function of lipotubuloids and microtubules in lipid synthesis and cuticle formation
    •  structure, function and transcriptional activity of nucleoli in soybean root meristematic cells under chilling stress and during recovery
    • the role of polysaccharides as well as of phosphorylation and dephosphorylation processes during cell cycle in   meristematic cells of Vicia faba and Allium cepa roots
    • mechanisms regulating sex determination and morphogenesis of gametophytes of Anemia phyllitidis fern
    • induction and mechanisms of programmed cell death in root cells of  Vicia faba
    • mechanisms of growth regulation and cell development mediated by plant hormones
    • chromatin, chromatids and chromosomes during prematurely induced mitosis and their activity during replication and nuclear division

 

  • Scientific projects/Grants

‘Asynchrony of G2-M transition under replication stress. Polarized course of chromatincondensation in Allium cepa root cells’; realization time 2010-2013, N N303 503038

‘Chromatin, chromatids and chromosomes during the course of prematurely induced mitoses and their behavior within the second round of the nuclear replication and division’, Project number POMOST/2011-4/8, realization time 2012-2015, Project co-financed by EU project under the Operational Program:  Innovative Economy, Priority I, operation 1.2., enhancement of scientific staff potential.

 ‘Involvement  of MAP kinase pathway in response to replication stress and DNA demage in Vicia faba’. realization time: 12.12.2011 – 11.01.2013, UMO-2011/01/N/NZ3/00146

 

  • Selected publications

Byczkowska A., Kunikowska A., Kaźmierczak A., 2012,  Determination of ACC-induced cell-programmed death in roots of Vicia faba ssp. minor seedlings by acridine orange and ethidium bromide staining. Protoplasma DOI: 10.1007/s 00709-012-0383-9

Kwiatkowska M., Popłońska K., Wojtczak A., Stępiński D., Polit J.T., 2012a Lipid body biogenesis and the role of microtubules in lipid synthesis in Ornithogalum umbellatum lipotubuloids. Cell Biol Int 36: 455-462

Kwiatkowska M., Popłońska K., Stępiński D., Wojtczak A., Polit J.T., Paszak K., 2012b Lipotubuloids – structure and function; in Advances in Selected Plant Physiology Aspects, Ed. Montanaro G. and Dichio B.; Intech pp 365-388

Polit J. T., Kaźmierczak A., Walczak-Drzewiecka A., 2012. Cell cycle-dependent phosphorylation of pRb-like protein in root meristem cells of Vicia faba. Protoplasma
249: 131-137.

Polit JT., Ciereszko I., 2012. Sucrose synthase activity and carbohydrates content in relation to phosphorylation status of Vicia faba root meristems during reactivation from sugar depletion. J Plant Physiol. DOI: 10.1016/j.jplph.2012.04.017.

Popłońska K., 2012 Occurence of calreticulin during the exchange of nucleohistones into protamine-type proteins in Chara vulgaris spermiogenesis. Protoplasma
DOI 10.1007/s00709-011-0370-6 (on line 2011)

Rybaczek D., Kowalewicz-Kulbat M., 2012, Relation of the types of DNA damage to replication stress and the induction of premature chromosome condensation. In: DNA Repair - New Research Directions (Ed. C. Chen) InTech - Open Access Publisher, Rijeka, Croatia ISBN: 980-953-307-746-3, pp 3-19.

Stępiński D., 2012, Levels of DNA methylation and histone methylation and acetylation change in root tip cells of soybean seedlings grown at different temperatures. Plant Physiology and Biochemistry 61: 9-17

Stępiński D., 2012, Nucleoar chromatin organization at different activities of soybean root meristematic cell nucleoli. Protoplasma DOI 10.1007/s00709-012-0456-9

Żabka A., Polit J.T., Maszewski J., 2012,DNA replication stress induces deregulation of the cell cycle events in root meristems of Allium cepa. Annals of Botany, DOI:10.1093/aob/mcs215

Winnicki K., Polit JT., Maszewski J., 2012, Increased transcription in hydroxyurea-treated root meristem cells of Vicia faba. Protoplasma

Winnicki K., 2012, Behavior of RNAs transcripts during nucleolus assembly and disassembly in Vicia faba root meristematic cells under normal conditions and after colchicine treatment. Acta Physiol Plant, DOI: 10.1007/s11738-012-0936-8

Rybaczek D., Kowalewicz-Kulbat M., 2012, Relation of the types of DNA damage to replication stress and the induction of premature chromosome condensation. In: DNA Repair - New Research Directions (Ed. C. Chen) InTech - Open Access Publisher, Rijeka, Croatia ISBN: 980-953-307-746-3, pp 3-19.

Rybaczek D., 2011, Eidetic analysis of the premature chromosome condensation process. In: DNA Repair (Ed. I. Kruman) InTech - Open Access Publisher, Rijeka, Croatia ISBN: 978-953-307-697-3, pp 185-204. 

Kaźmierczak A., 2010., Endoreplication in Anemia phyllitidis coincides with the development of gametophytes and male sex. Physiol Plant 138: 321–328

Kaźmierczak A., 2010, Gibberellic acid and ethylene control male sex determination and development of Anemia phyllitidis gametophytes. W: Fernandez H, Revilla MA, Kumar A (red), Working with Ferns. Issue and Applications, ss 49-66.

Kwiatkowska M., Stępiński D., Popłońska K., Wojtczak A., Polit J.T., 2010 „Elaioplasts” of Haemanthus albiflos are true lipotubuloids: cytoplasmic domains rich in lipid bodies entwined by microtubules. Acta Physiol Plant 32:1189-1196

Stępiński D., 2010, Organization of the nucleoli of soybean root meristematic cells at different states of their activity. Micron 41:283-288

Żabka A., Polit J.T., Maszewski J., 2010. Inter- and intrachromosomal asynchrony of cell division cycle events in root meristem cells of Allium cepa: possible connection with gradient of cyclin B-like proteins. Plant Cell Reports  29: 845–856.

 

  • Theses topics

Master’s theses

 ‘The role of endoplasmic reticulum in growth of Anemia phyllitidis fern gametophytes’.

 The role of kinetin in long-lasting growth of Vicia faba ssp. Minor’.

‘Programmed cell death (PCD) induction in roots of seedlings of Vicia faba ssp. minor using kinetin’.

 ‘Influence of replication stress on mitotic processes in root meristem cells of Allium cepa’.

‘Induction of premature chromosome condensation (PCC) in root meristem cells of Allium cepa treated with hydroxyurea and caffeine’.

Bachelor’s theses

 ‘Basic mechanisms controlling the number of cells in eukaryotic organisms’.

‘Cell cycle and cell death as the basic mechanisms regulating the number of cells in eukaryotic organisms’.

 ‘Endoreduplication in scientific papers in Department of Cytophysiology, University of Łódź.

‘Noninvasive methods for evaluation of human oocytes and embryos’.

‘From one cell to cancer’.

‘Endosymbiotic origin of mitochondria and chloroplasts’.